-Dissolve 40.01 grams in 1000ml distilled water.
-Gently heat to boiling with swirling to dissolve the medium completely.
-Dispense into test tubes with Durham’s tube.
-Sterilize by autoclaving at 15 psi (121oC) for 15 minutes.
-Cool the tubes to 45
-Dissolve 40.01 grams in 1000ml distilled water.
-Gently heat to boiling with swirling to dissolve the medium completely.
-Dispense into test tubes with Durham’s tube.
-Sterilize by autoclaving at 15 psi (121oC) for 15 minutes.
-Cool the tubes to 45
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