-Dissolve 63.0 grams in 950 ml purified/ distilled water.
-Heat to boiling to dissolve the medium completely.
-Sterilize by autoclaving at 15 psi pressure (121°C) for 15 minutes.
-Cool to 50°C and aseptically add 50 ml concentrated Egg Yolk Emulsion
-Dissolve 63.0 grams in 950 ml purified/ distilled water.
-Heat to boiling to dissolve the medium completely.
-Sterilize by autoclaving at 15 psi pressure (121°C) for 15 minutes.
-Cool to 50°C and aseptically add 50 ml concentrated Egg Yolk Emulsion
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