-Dissolve 40.0 grams in 1000 ml purified/distilled water. Add 2 grams of D, 0.025 grams Bromothymol blue and 10 grams of mannitol.
-Heat to boiling to dissolve the medium completely.
-Sterilize by autoclaving at 12 to 15 psi pressure (118°C to 121°C)
-Dissolve 40.0 grams in 1000 ml purified/distilled water. Add 2 grams of D, 0.025 grams Bromothymol blue and 10 grams of mannitol.
-Heat to boiling to dissolve the medium completely.
-Sterilize by autoclaving at 12 to 15 psi pressure (118°C to 121°C)
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