-Dissolve the 37.5 grams in 1000 ml distilled water.
-Heat to boiling to dissolve the medium completely.
-Sterilize by autoclaving at 15 psi pressure (121°C) for 15 minutes. Avoid overheating.
-Cool to 50°C and shake the medium in order to oxidize th
-Dissolve the 37.5 grams in 1000 ml distilled water.
-Heat to boiling to dissolve the medium completely.
-Sterilize by autoclaving at 15 psi pressure (121°C) for 15 minutes. Avoid overheating.
-Cool to 50°C and shake the medium in order to oxidize th
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