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Emb Agar
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MLSIGNUP100
Expiring in 24days 16hr10min11sec
-Dissolve 35.96 grams in 1000 ml purified / distilled water.
-Mix until suspension is uniform. Heat to boiling to dissolve the medium completely.
-Sterilize by autoclaving at 15 psi pressure (121°C) for 15 minutes. AVOID OVERHEATING.
-Cool to 45-50°C and shake the medium in order to oxidize the methylene blue (i.e. to restore its blue colour) and to suspend the flocculent precipitate. (If EMB Agar is inoculated on the same day, it may be used without autoclave sterilization).
-Dissolve 35.96 grams in 1000 ml purified / distilled water.
-Mix until suspension is uniform. Heat to boiling to dissolve the medium completely.
-Sterilize by autoclaving at 15 psi pressure (121°C) for 15 minutes. AVOID OVERHEATING.
-Cool to 45-50°C and shake the medium in order to oxidize the methylene blue (i.e. to restore its blue colour) and to suspend the flocculent precipitate. (If EMB Agar is inoculated on the same day, it may be used without autoclave sterilization).
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