-Dissolve 8.75 grams in 250 ml purified/distilled water.
-Heat if necessary to dissolve the medium completely.
-Sterilize by autoclaving at 10psi pressure (115°C) for 20 minutes.
-Cool to 45-50°C and aseptically add 750 ml of sterile Horse Serum.
-M
-Dissolve 8.75 grams in 250 ml purified/distilled water.
-Heat if necessary to dissolve the medium completely.
-Sterilize by autoclaving at 10psi pressure (115°C) for 20 minutes.
-Cool to 45-50°C and aseptically add 750 ml of sterile Horse Serum.
-M
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