-Dissolve 37.24 grams in 600 ml purified / distilled water containing 12 ml glycerol (for bovine bacteria or other glycerophobic organisms additions of glycerol is not desirable).
-Heat if necessary to dissolve the medium completely.
-Sterilize by autoc
-Dissolve 37.24 grams in 600 ml purified / distilled water containing 12 ml glycerol (for bovine bacteria or other glycerophobic organisms additions of glycerol is not desirable).
-Heat if necessary to dissolve the medium completely.
-Sterilize by autoc
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