-Dissolve 55.07 grams in 1000 ml distilled water.
-Heat to boiling with gentle swirling to dissolve the medium completely.
-Sterilize by autoclaving at 15 psi pressure (121°C) for 15 minutes. Avoid overheating.
-Cool to 45-50°C and pour into sterile
-Dissolve 55.07 grams in 1000 ml distilled water.
-Heat to boiling with gentle swirling to dissolve the medium completely.
-Sterilize by autoclaving at 15 psi pressure (121°C) for 15 minutes. Avoid overheating.
-Cool to 45-50°C and pour into sterile
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