-Dissolve 18.5 grams in 500 ml purified / distilled water.
-Heat to boiling to dissolve the medium completely.
-Sterilize by autoclaving at 15 psi pressure (121°C) for 15 minutes.
-Cool to 45-50°C and aseptically add reconstituted contents of one via
-Dissolve 18.5 grams in 500 ml purified / distilled water.
-Heat to boiling to dissolve the medium completely.
-Sterilize by autoclaving at 15 psi pressure (121°C) for 15 minutes.
-Cool to 45-50°C and aseptically add reconstituted contents of one via
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