-Dissolve 30.26 grams in 1000 ml distilled water.
-Heat if necessary to dissolve the medium completely.
-Dispense in 100 ml amounts in flasks. Sterilize by autoclaving at 15 psi pressure (121°C) for 15 minutes.
-Cool to 45-50°C and aseptically add 5%
-Dissolve 30.26 grams in 1000 ml distilled water.
-Heat if necessary to dissolve the medium completely.
-Dispense in 100 ml amounts in flasks. Sterilize by autoclaving at 15 psi pressure (121°C) for 15 minutes.
-Cool to 45-50°C and aseptically add 5%
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